Hotspot

rpl32-trnL-UAG

SSC · 115186-115963 · 778 bp

Back to Rubus

Pi 0.0439
MarkerSeek score 78.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
778
variable_sites
258
pi
0.0439
unique_haplotype_count
72
primer_available
yes
alignment_reliability
0.93
markerseek_score
78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TCTCTTTCTACCGGTAGCTCA AGCGTGTCTACCGATTTCAC 47.6 50.0 57.6 58.0 747-881 bp 0.988235 80.6
rpl32-trnL-UAG_p2 TCTCTTTCTACCGGTAGCTCA CAGCGTGTCTACCGATTTCA 47.6 50.0 57.6 58.0 748-882 bp 0.988235 80.6
rpl32-trnL-UAG_p3 TCTCTTTCTACCGGTAGCTCA TCCTAAGAGCAGCGTGTCTA 47.6 50.0 57.6 57.9 757-891 bp 0.988235 79.6
rpl32-trnL-UAG_p4 TCTCTTTCTACCGGTAGCTCA CCTAAGAGCAGCGTGTCTAC 47.6 55.0 57.6 57.8 756-890 bp 0.988235 79.1
rpl32-trnL-UAG_p5 TCTCTTTCTACCGGTAGCTCA CTAAGAGCAGCGTGTCTACC 47.6 55.0 57.6 57.8 755-889 bp 0.988235 79.1