Hotspot

ndhF-rpl32

SSC · 113925-115026 · 1102 bp

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Pi 0.0281
MarkerSeek score 69.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1102
variable_sites
268
pi
0.0281
unique_haplotype_count
67
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CACTCGGAAAAGCCCACATA TCGATGGGGAAGGTGAGAAT 50.0 50.0 57.9 58.1 1542-1886 bp 0.976471 89.2
ndhF-rpl32_p2 ACTCGGAAAAGCCCACATAC TCGATGGGGAAGGTGAGAAT 50.0 50.0 57.9 58.1 1541-1885 bp 0.976471 89.2
ndhF-rpl32_p3 CACTCGGAAAAGCCCACATA GAAACCTTGTGCTTGCGTTT 50.0 45.0 57.9 58.1 1482-1826 bp 0.682353 77.8
ndhF-rpl32_p4 ACTCGGAAAAGCCCACATAC GAAACCTTGTGCTTGCGTTT 50.0 45.0 57.9 58.1 1481-1825 bp 0.682353 77.8
ndhF-rpl32_p5 ATGAAGGGGAAATTGGGCAG GAAACCTTGTGCTTGCGTTT 50.0 45.0 58.1 58.1 2090-2434 bp 0.658824 76.7