Hotspot

rpl22-rps19

LSC · 85475-85574 · 100 bp

Back to Rubus

Pi 0.05
MarkerSeek score 67.60
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
100
variable_sites
42
pi
0.05
unique_haplotype_count
49
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 GTCGTACCCTCGTTGACTTC TCCCGAGCGTCTACCATTAT 55.0 50.0 58.0 58.0 525-590 bp 1.0 91.7
rpl22-rps19_p2 CGTACCCTCGTTGACTTCAG TCCCGAGCGTCTACCATTAT 55.0 50.0 58.0 58.0 523-588 bp 0.976471 90.8
rpl22-rps19_p3 TTGTCCCATAATCCCCCACT TCCCGAGCGTCTACCATTAT 50.0 50.0 58.0 58.0 817-882 bp 0.988235 90.3
rpl22-rps19_p4 TTGTCCCATAATCCCCCACT GGGCCCCAATTGGTAGAAAA 50.0 50.0 58.0 58.1 1099-1244 bp 0.988235 89.5
rpl22-rps19_p5 ATACGTGCAATTCCCTCGAC TCCCGAGCGTCTACCATTAT 50.0 50.0 58.1 58.0 996-1061 bp 0.988235 89.4