Hotspot

petA-psbJ

LSC · 64226-65208 · 983 bp

Back to Rubus

Pi 0.0357
MarkerSeek score 74.80
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
983
variable_sites
239
pi
0.0357
unique_haplotype_count
63
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TGAGAAGGTTCAATTGTCCGA ACCGGTATTGTTGTGATTGGT 42.9 42.9 57.2 57.9 811-1206 bp 0.988235 68.2
petA-psbJ_p2 TGAGAAGGTTCAATTGTCCGAA ACCGGTATTGTTGTGATTGGT 40.9 42.9 57.9 57.9 811-1206 bp 0.988235 65.7
petA-psbJ_p3 TTGAGAAGGTTCAATTGTCCGA ACCGGTATTGTTGTGATTGGT 40.9 42.9 57.9 57.9 812-1207 bp 0.988235 65.7
petA-psbJ_p4 TGAGAAGGTTCAATTGTCCGA GCCGATACTACTGGAAGGAT 42.9 50.0 57.2 56.2 856-1251 bp 0.988235 63.1
petA-psbJ_p5 TGAGAAGGTTCAATTGTCCG ACCGGTATTGTTGTGATTGGT 45.0 42.9 55.7 57.9 811-1206 bp 0.964706 62.9