Hotspot

petN-psbM

LSC · 28509-29790 · 1282 bp

Back to Rubus

Pi 0.0273
MarkerSeek score 77.20
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1282
variable_sites
280
pi
0.0273
unique_haplotype_count
66
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 AGTAAATCTCGCTTGGGCTG AAAACAGTCAGCCAAGGTGA 50.0 45.0 58.0 57.6 1350-1455 bp 0.976471 87.3
petN-psbM_p2 AGTAAATCTCGCTTGGGCTG AAACAGTCAGCCAAGGTGAT 50.0 45.0 58.0 57.3 1349-1454 bp 0.976471 85.5
petN-psbM_p3 AGTAAATCTCGCTTGGGCTG AACAGTCAGCCAAGGTGATT 50.0 45.0 58.0 57.3 1348-1453 bp 0.976471 85.5
petN-psbM_p4 ATGGGGAAGAAGTGGACTCT AAAACAGTCAGCCAAGGTGA 50.0 45.0 57.6 57.6 1290-1395 bp 0.976471 84.8
petN-psbM_p5 ATGGGGAAGAAGTGGACTCT AACAGTCAGCCAAGGTGATT 50.0 45.0 57.6 57.3 1288-1393 bp 0.976471 82.9