Hotspot

trnC-GCA-petN

LSC · 27649-28418 · 770 bp

Back to Rubus

Pi 0.0177
MarkerSeek score 68.00
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
770
variable_sites
154
pi
0.0177
unique_haplotype_count
54
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG CAGCCCAAGCGAGATTTACT 50.0 50.0 58.1 58.0 813-847 bp 0.988235 90.6
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.1 57.6 873-907 bp 1.0 88.5
trnC-GCA-petN_p3 GGGGGACTGCAAATCCTTTT CAGCCCAAGCGAGATTTACT 50.0 50.0 58.4 58.0 831-865 bp 0.988235 88.4
trnC-GCA-petN_p4 GGGGGACTGCAAATCCTTTT AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.4 57.6 891-925 bp 1.0 86.3
trnC-GCA-petN_p5 CCAGTTCAAATCCGGGTGTC CAGCCCAAGCGAGATTTACT 55.0 50.0 58.8 58.0 808-842 bp 0.988235 84.6