Hotspot

ycf1

SSC · 107768-113341 · 5574 bp

Back to Rubroshorea

Pi 0.0273

MarkerSeek score 53.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5574
variable_sites: 152
pi: 0.0273
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTTGCGGGACAGCTCATAA	CCAAAAGTTGCCAAACTGGG	45.0	50.0	57.8	58.0	1155-1203 bp	1.0	81.9
ycf1_p2	TTTTTGCGGGACAGCTCATA	CCAAAAGTTGCCAAACTGGG	45.0	50.0	57.8	58.0	1156-1204 bp	1.0	81.9
ycf1_p3	TTTTGCGGGACAGCTCATAA	ATGGTCGTCTCCTTCGGTTA	45.0	50.0	57.8	58.2	1022-1070 bp	1.0	81.1
ycf1_p4	TTTTGCGGGACAGCTCATAA	TGGTCGTCTCCTTCGGTTAT	45.0	50.0	57.8	58.2	1021-1069 bp	1.0	81.1
ycf1_p5	TTTTTGCGGGACAGCTCATA	TGGTCGTCTCCTTCGGTTAT	45.0	50.0	57.8	58.2	1022-1070 bp	1.0	81.1