Hotspot

trnN-GUU-ycf1

IRb · 107329-107767 · 439 bp

Back to Rubroshorea

Pi 0.052
MarkerSeek score 74.30
Primer available yes
Label trnN-GUU-ycf1

Feature Metadata

label_name
trnN-GUU-ycf1
feature_type
igs
region
IRb
length_bp
439
variable_sites
22
pi
0.052
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ycf1_p1 AGGATTCGAACCTACGACCA ATTACCTGGTATAAACGGCA 50.0 40.0 58.2 54.1 521-522 bp 1.0 53.6
trnN-GUU-ycf1_p2 AGGATTCGAACCTACGACCA TTACCTGGTATAAACGGCAT 50.0 40.0 58.2 54.1 520-521 bp 1.0 53.6
trnN-GUU-ycf1_p3 CCCCAAGTAGGATTCGAACC ATTACCTGGTATAAACGGCA 55.0 40.0 57.7 54.1 529-530 bp 1.0 52.1
trnN-GUU-ycf1_p4 CCCCAAGTAGGATTCGAACC TTACCTGGTATAAACGGCAT 55.0 40.0 57.7 54.1 528-529 bp 1.0 52.1
trnN-GUU-ycf1_p5 AGGATTCGAACCTACGACCA ATTACCTGGTATAAACGGCAT 50.0 38.1 58.2 54.8 521-522 bp 1.0 50.7