Hotspot

rps12-psbB

LSC · 71158-73169 · 2012 bp

Back to Rubroshorea

Pi 0.0234

MarkerSeek score 66.30

Primer available yes

Label rps12-psbB

Feature Metadata

label_name: rps12-psbB
feature_type: igs
region: LSC
length_bp: 2012
variable_sites: 46
pi: 0.0234
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-psbB_p1	GATTGGCTGTCTTGCGTTTC	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	2055-2077 bp	1.0	81.4
rps12-psbB_p2	CTCGTACATGTTCCTCGACG	CGATACCAAGGCAAACCCAT	55.0	50.0	58.2	58.2	2117-2139 bp	1.0	80.1
rps12-psbB_p3	CCTCGTACATGTTCCTCGAC	CGATACCAAGGCAAACCCAT	55.0	50.0	57.8	58.2	2118-2140 bp	1.0	80.0
rps12-psbB_p4	AGGACATCCCCTAAGAGCAG	CGATACCAAGGCAAACCCAT	55.0	50.0	58.2	58.2	2094-2116 bp	1.0	80.0
rps12-psbB_p5	GAGGACATCCCCTAAGAGCA	CGATACCAAGGCAAACCCAT	55.0	50.0	58.2	58.2	2095-2117 bp	1.0	80.0