Hotspot

rpl33-rps18

LSC · 68786-69324 · 539 bp

Back to Rubroshorea

Pi 0.0342

MarkerSeek score 65.20

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 539
variable_sites: 16
pi: 0.0342
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGGTTCAGGTTCCAAGACAA	CTTTCTCAGCAGGAATCCCC	50.0	55.0	58.0	58.0	1605-1684 bp	1.0	85.2
rpl33-rps18_p2	CGGTTCAGGTTCCAAGACAA	TGTAGTTCTAGCGGTCGACT	50.0	50.0	58.0	57.9	1335-1393 bp	1.0	84.9
rpl33-rps18_p3	AGGGGATTCATGGCCAAAAG	CTTTCTCAGCAGGAATCCCC	50.0	55.0	58.1	58.0	1280-1358 bp	1.0	84.7
rpl33-rps18_p4	CGGTTCAGGTTCCAAGACAA	GAACTCGGGGAAGGTAGAGT	50.0	55.0	58.0	58.2	1557-1636 bp	1.0	84.4
rpl33-rps18_p5	CGGTTCAGGTTCCAAGACAA	TGAACTCGGGGAAGGTAGAG	50.0	55.0	58.0	58.2	1558-1637 bp	1.0	84.4