Hotspot

psbE-petL

LSC · 65542-66643 · 1102 bp

Back to Rubroshorea

Pi 0.0306
MarkerSeek score 68.30
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1102
variable_sites
33
pi
0.0306
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AGAACGTTCTCCTGTGCTTC TGAGGTTATAGTTAAAGCCGCT 50.0 40.9 57.8 57.3 1181-1186 bp 1.0 60.6
psbE-petL_p2 GTTCTCCTGTGCTTCCAGAC TGAGGTTATAGTTAAAGCCGCT 55.0 40.9 58.2 57.3 1176-1181 bp 1.0 60.3
psbE-petL_p3 ACGAATAACCAACCCGCAAT TGAGGTTATAGTTAAAGCCGCT 45.0 40.9 58.2 57.3 1267-1272 bp 1.0 60.2
psbE-petL_p4 TGCTGACGAATAACCAACCC TGAGGTTATAGTTAAAGCCGCT 50.0 40.9 58.2 57.3 1272-1277 bp 1.0 60.1
psbE-petL_p5 CGAATAACCAACCCGCAATG TGAGGTTATAGTTAAAGCCGCT 50.0 40.9 57.8 57.3 1266-1271 bp 1.0 60.1