Hotspot

psbZ-trnG-GCC

LSC · 36218-36826 · 609 bp

Back to Rubroshorea

Pi 0.0741

MarkerSeek score 94.70

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 609
variable_sites: 43
pi: 0.0741
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 94.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGGTG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	57.9	57.8	763-839 bp	1.0	84.9
psbZ-trnG-GCC_p2	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	766-842 bp	1.0	84.2
psbZ-trnG-GCC_p3	TTCTCCTGATGGTTGGGTGA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.3	57.8	762-838 bp	1.0	83.8
psbZ-trnG-GCC_p4	CTTCTCCTGATGGTTGGGTG	TAAATACATTGCCTGGGCGG	55.0	50.0	57.9	58.3	802-878 bp	1.0	83.4
psbZ-trnG-GCC_p5	TCCTGATGGTTGGGTGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.7	57.8	759-835 bp	1.0	83.2