Hotspot

psbC-trnS-UGA

LSC · 35369-35607 · 239 bp

Back to Rubroshorea

Pi 0.0669

MarkerSeek score 75.90

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 239
variable_sites: 16
pi: 0.0669
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TCGTGGGTCATTTATGGCAC	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.3	58.1	421-435 bp	1.0	80.5
psbC-trnS-UGA_p2	AGCGGGATTTGAAAAGGGAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.7	58.1	378-392 bp	1.0	80.4
psbC-trnS-UGA_p3	TCGTGGGTCATTTATGGCAC	ACCGGCATAGTTCGAAGAAC	50.0	50.0	58.3	58.0	398 bp	0.5	57.9
psbC-trnS-UGA_p4	AGCGGGATTTGAAAAGGGAA	ACCGGCATAGTTCGAAGAAC	45.0	50.0	57.7	58.0	355 bp	0.5	57.5
psbC-trnS-UGA_p5	TCGTGATCTTGAGCCTGTTC	ACCGGCATAGTTCGAAGAAC	50.0	50.0	57.6	58.0	331 bp	0.5	57.0