Hotspot

atpF-atpH

LSC · 12906-13492 · 587 bp

Back to Rubroshorea

Pi 0.0363

MarkerSeek score 80.80

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 587
variable_sites: 21
pi: 0.0363
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCAGTGACCCAAAGAAACGA	CCCAGGCGGATGGAAAAATA	50.0	50.0	58.0	57.9	748-779 bp	1.0	82.3
atpF-atpH_p2	CCAGTGACCCAAAGAAACGA	TGGTTATCGCATTGGCACTT	50.0	45.0	58.0	58.2	673-704 bp	1.0	81.8
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	CCCAGGCGGATGGAAAAATA	50.0	50.0	57.9	57.9	775-806 bp	1.0	81.6
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	TGGTTATCGCATTGGCACTT	50.0	45.0	57.9	58.2	700-731 bp	1.0	81.1
atpF-atpH_p5	CCAGTGACCCAAAGAAACGA	CTGGTTATCGCATTGGCACT	50.0	50.0	58.0	58.6	674-705 bp	1.0	78.2