Hotspot

trnS-GCU-trnG-UCC

LSC · 9037-9628 · 592 bp

Back to Rubroshorea

Pi 0.0451
MarkerSeek score 80.40
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
592
variable_sites
26
pi
0.0451
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 AGCAAAACGGAAAGAGAGGG CGAAGGACCCCTTAACTATCA 50.0 47.6 58.1 56.5 756-768 bp 1.0 63.9
trnS-GCU-trnG-UCC_p2 GCAAAACGGAAAGAGAGGGA CGAAGGACCCCTTAACTATCA 50.0 47.6 58.1 56.5 755-767 bp 1.0 63.8
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA CGAAGGACCCCTTAACTATCA 50.0 47.6 58.2 56.5 750-762 bp 1.0 63.5
trnS-GCU-trnG-UCC_p4 AGCAAAACGGAAAGAGAGGG CGAAGGACCCCTTAACTATCAA 50.0 45.5 58.1 57.3 756-768 bp 1.0 61.6
trnS-GCU-trnG-UCC_p5 GCAAAACGGAAAGAGAGGGA CGAAGGACCCCTTAACTATCAA 50.0 45.5 58.1 57.3 755-767 bp 1.0 61.6