Hotspot

ndhA

SSC · 120547-122820 · 2274 bp

Back to Rubia

Pi 0.00611
MarkerSeek score 44.40
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2274
variable_sites
39
pi
0.00611
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
44.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 TCGCATGAGCTAAGGCAATT TGATAGGAGATCCAGGCGTT 45.0 50.0 58.0 57.9 2619-2637 bp 1.0 81.0
ndhA_p2 TCGCATGAGCTAAGGCAATT TTGATAGGAGATCCAGGCGT 45.0 50.0 58.0 57.9 2620-2638 bp 1.0 81.0
ndhA_p3 TTCGCATGAGCTAAGGCAAT TGATAGGAGATCCAGGCGTT 45.0 50.0 58.0 57.9 2620-2638 bp 1.0 81.0
ndhA_p4 ACCGACCCAACGAAATTTGA TGATAGGAGATCCAGGCGTT 45.0 50.0 58.0 57.9 2952-2970 bp 1.0 80.9
ndhA_p5 ACCGACCCAACGAAATTTGA TTGATAGGAGATCCAGGCGT 45.0 50.0 58.0 57.9 2953-2971 bp 1.0 80.9