Hotspot

rpl36-rps8

LSC · 80197-80795 · 599 bp

Back to Rubia

Pi 0.0132
MarkerSeek score 66.60
Primer available yes
Label rpl36-rps8

Feature Metadata

label_name
rpl36-rps8
feature_type
igs
region
LSC
length_bp
599
variable_sites
22
pi
0.0132
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl36-rps8_p1 TCCCTGCCTTTGCTTATGTC GCGGGATGGGGATTGTAATT 50.0 50.0 57.9 58.0 724-896 bp 1.0 85.1
rpl36-rps8_p2 CCCTGCCTTTGCTTATGTCT GCGGGATGGGGATTGTAATT 50.0 50.0 57.9 58.0 723-895 bp 1.0 85.1
rpl36-rps8_p3 TCCCTGCCTTTGCTTATGTC TTCCTCGAATTTTAGGCGGG 50.0 50.0 57.9 58.0 739-911 bp 1.0 84.9
rpl36-rps8_p4 TCCCTGCCTTTGCTTATGTC TCGAATTTTAGGCGGGATGG 50.0 50.0 57.9 58.0 735-907 bp 1.0 84.8
rpl36-rps8_p5 CCCTGCCTTTGCTTATGTCT TTCCTCGAATTTTAGGCGGG 50.0 50.0 57.9 58.0 738-910 bp 1.0 84.8