Hotspot

psaJ-rps18

LSC · 67646-68487 · 842 bp

Back to Rubia

Pi 0.0177
MarkerSeek score 57.50
Primer available yes
Label psaJ-rps18

Feature Metadata

label_name
psaJ-rps18
feature_type
igs
region
LSC
length_bp
842
variable_sites
31
pi
0.0177
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rps18_p1 GGATGCGTTAACATTCCCCT ACGCCTACGAAAAGATTGCT 50.0 45.0 57.9 57.9 926-937 bp 1.0 82.7
psaJ-rps18_p2 GGATGCGTTAACATTCCCCT CAATTCGATCCCCCGATTGA 50.0 50.0 57.9 57.7 957-968 bp 1.0 81.3
psaJ-rps18_p3 GGATGCGTTAACATTCCCCT TCAATTCGATCCCCCGATTG 50.0 50.0 57.9 57.7 958-969 bp 1.0 81.3
psaJ-rps18_p4 TCCGGATGCGTTAACATTCC ACGCCTACGAAAAGATTGCT 50.0 45.0 58.3 57.9 929-940 bp 1.0 80.4
psaJ-rps18_p5 TCCGGATGCGTTAACATTCC CAATTCGATCCCCCGATTGA 50.0 50.0 58.3 57.7 960-971 bp 1.0 79.0