Hotspot

trnP-UGG-psaJ

LSC · 67135-67510 · 376 bp

Back to Rubia

Pi 0.0119
MarkerSeek score 38.50
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
376
variable_sites
15
pi
0.0119
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
38.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 AAAACAAACGCGCTACCAAG GCTAAAGCCACGAACCAAAG 45.0 50.0 57.9 57.7 479-502 bp 1.0 78.9
trnP-UGG-psaJ_p2 AAAACAAACGCGCTACCAAG AAGCCACGAACCAAAGTGTA 45.0 45.0 57.9 57.7 475-498 bp 1.0 78.9
trnP-UGG-psaJ_p3 CAAAACAAACGCGCTACCAA GCTAAAGCCACGAACCAAAG 45.0 50.0 58.2 57.7 480-503 bp 1.0 78.5
trnP-UGG-psaJ_p4 CAAAACAAACGCGCTACCAA AAGCCACGAACCAAAGTGTA 45.0 45.0 58.2 57.7 476-499 bp 1.0 78.5
trnP-UGG-psaJ_p5 CCTCAGGTGATCCCCCATTA GCTAAAGCCACGAACCAAAG 55.0 50.0 58.2 57.7 561-584 bp 1.0 78.0