Hotspot

trnT-GGU-psbD

LSC · 32204-33556 · 1353 bp

Back to Rubia

Pi 0.00826
MarkerSeek score 58.20
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1353
variable_sites
32
pi
0.00826
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GGACCAGCCTACAAAAACGA 55.0 50.0 58.1 58.1 1491-1513 bp 1.0 84.1
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GGACCAGCCTACAAAAACGA 50.0 50.0 58.1 58.1 1494-1516 bp 1.0 83.9
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG ACGGTCTCTACGTAACCAGT 55.0 50.0 58.1 57.8 1470-1492 bp 1.0 83.6
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA ACGGTCTCTACGTAACCAGT 50.0 50.0 58.1 57.8 1473-1495 bp 1.0 83.4
trnT-GGU-psbD_p5 GGCGTAAGTCATCGGTTCAA GGACCAGCCTACAAAAACGA 50.0 50.0 58.3 58.1 1468-1490 bp 1.0 82.5