Hotspot

trnE-UUC-trnT-GGU

LSC · 31318-32131 · 814 bp

Back to Rubia

Pi 0.00527
MarkerSeek score 52.90
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
814
variable_sites
14
pi
0.00527
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CGTAGTATCCTACCCCCAGG GAACCGATGACTTACGCCTT 60.0 50.0 58.1 58.0 940-971 bp 1.0 81.7
trnE-UUC-trnT-GGU_p2 CGTAGTATCCTACCCCCAGG CCATGGCGTTACTCTACCAC 60.0 55.0 58.1 58.1 919-950 bp 1.0 81.3
trnE-UUC-trnT-GGU_p3 CGTAGTATCCTACCCCCAGG TGGCGTTACTCTACCACTGA 60.0 50.0 58.1 58.1 916-947 bp 1.0 81.1
trnE-UUC-trnT-GGU_p4 CGTAGTATCCTACCCCCAGG AATCAGAGCCCCTTATCGGA 60.0 50.0 58.1 57.9 963-994 bp 1.0 80.8
trnE-UUC-trnT-GGU_p5 CGTAGTATCCTACCCCCAGG TCAGAGCCCCTTATCGGATT 60.0 50.0 58.1 57.9 961-992 bp 1.0 80.8