Hotspot

psbM-trnD-GUC

LSC · 30215-30920 · 706 bp

Back to Rubia

Pi 0.014
MarkerSeek score 55.80
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
706
variable_sites
29
pi
0.014
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 CCATTCGATTCCGATGGAGG TAACCCCCTTGCCCTTCATA 55.0 50.0 58.2 58.0 1501-1583 bp 1.0 84.7
psbM-trnD-GUC_p2 CCATTCGATTCCGATGGAGG TATAACCCCCTTGCCCTTCA 55.0 50.0 58.2 58.0 1503-1585 bp 1.0 84.7
psbM-trnD-GUC_p3 CCATTCGATTCCGATGGAGG TATGATGGCGGTTGGACAAG 55.0 50.0 58.2 58.0 1769-1851 bp 1.0 84.2
psbM-trnD-GUC_p4 CCATTCGATTCCGATGGAGG CCTACGCTGGTTCAAATCCA 55.0 50.0 58.2 57.9 1560-1642 bp 1.0 83.9
psbM-trnD-GUC_p5 CCATTCGATTCCGATGGAGG ATGCCTACGCTGGTTCAAAT 55.0 45.0 58.2 57.9 1563-1645 bp 1.0 83.7