Hotspot

rpoB-trnC-GCA

LSC · 27665-28735 · 1071 bp

Back to Rubia

Pi 0.00783
MarkerSeek score 55.20
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1071
variable_sites
25
pi
0.00783
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CATTCCCTCGTTTCCATCCC CCGGATTTGAACTGGGGAAA 55.0 50.0 58.3 58.1 1142-1176 bp 1.0 81.3
rpoB-trnC-GCA_p2 ACATTCCCTCGTTTCCATCC CCGGATTTGAACTGGGGAAA 50.0 50.0 57.6 58.1 1143-1177 bp 1.0 80.4
rpoB-trnC-GCA_p3 TCGTTTCCATCCCCTAGCAT CCGGATTTGAACTGGGGAAA 50.0 50.0 58.5 58.1 1135-1169 bp 1.0 79.9
rpoB-trnC-GCA_p4 CATTCCCTCGTTTCCATCCC AAAAGGATTTGCAGTCCCCC 55.0 50.0 58.3 58.4 1124-1158 bp 1.0 79.1
rpoB-trnC-GCA_p5 ACATTCCCTCGTTTCCATCC AAAAGGATTTGCAGTCCCCC 50.0 50.0 57.6 58.4 1125-1159 bp 1.0 78.3