Hotspot

trnS-GCU-trnS-CGA

LSC · 8759-9676 · 918 bp

Back to Rubia

Pi 0.0151
MarkerSeek score 62.60
Primer available yes
Label trnS-GCU-trnS-CGA

Feature Metadata

label_name
trnS-GCU-trnS-CGA
feature_type
igs
region
LSC
length_bp
918
variable_sites
38
pi
0.0151
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnS-CGA_p1 CAATCCGACGCTTTAGTCCA AAACCGAAAGCAAAGACCCT 50.0 45.0 58.0 57.9 698-1074 bp 1.0 86.6
trnS-GCU-trnS-CGA_p2 CAATCCGACGCTTTAGTCCA AACCGAAAGCAAAGACCCTT 50.0 45.0 58.0 57.9 697-1073 bp 1.0 86.6
trnS-GCU-trnS-CGA_p3 CAATCCGACGCTTTAGTCCA ACCGAAAGCAAAGACCCTTT 50.0 45.0 58.0 57.9 696-1072 bp 1.0 86.6
trnS-GCU-trnS-CGA_p4 AATCCGACGCTTTAGTCCAC AACCGAAAGCAAAGACCCTT 50.0 45.0 58.0 57.9 696-1072 bp 1.0 86.6
trnS-GCU-trnS-CGA_p5 AATCCGACGCTTTAGTCCAC ACCGAAAGCAAAGACCCTTT 50.0 45.0 58.0 57.9 695-1071 bp 1.0 86.6