Hotspot

trnK-UUU-rps16

LSC · 4153-4788 · 636 bp

Back to Rubia

Pi 0.0114
MarkerSeek score 59.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
636
variable_sites
18
pi
0.0114
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCACTTAAAAGCCGAG TGCTCAACTTACAGGAACCG 50.0 50.0 58.6 57.8 744-786 bp 1.0 79.0
trnK-UUU-rps16_p2 AGTCGCACTTAAAAGCCGAG GCTCAACTTACAGGAACCGT 50.0 50.0 58.6 57.8 743-785 bp 1.0 79.0
trnK-UUU-rps16_p3 TCGCACTTAAAAGCCGAGTA TGCTCAACTTACAGGAACCG 45.0 50.0 57.3 57.8 742-784 bp 1.0 77.8
trnK-UUU-rps16_p4 TCGCACTTAAAAGCCGAGTA GCTCAACTTACAGGAACCGT 45.0 50.0 57.3 57.8 741-783 bp 1.0 77.8
trnK-UUU-rps16_p5 TAGTCGCACTTAAAAGCCGA TGCTCAACTTACAGGAACCG 45.0 50.0 57.3 57.8 745-787 bp 1.0 77.7