Hotspot

trnH-GUG-psbA

LSC · 97-320 · 224 bp

Back to Rubia

Pi 0.0254
MarkerSeek score 57.40
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
224
variable_sites
14
pi
0.0254
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG AGCTGCTATAGATGCTCCATC 55.0 47.6 58.1 57.1 277-287 bp 1.0 70.2
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG TCTAGCTGCTATAGATGCTCCA 55.0 45.5 58.1 58.0 280-290 bp 1.0 68.9
trnH-GUG-psbA_p3 GATCCACTTGGCTACATCCG AGCTGCTATAGATGCTCCAT 55.0 45.0 58.1 55.9 277-287 bp 1.0 68.1
trnH-GUG-psbA_p4 GCCTTGATCCACTTGGCTAC AGCTGCTATAGATGCTCCATC 55.0 47.6 58.6 57.1 282-292 bp 1.0 66.1
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG GCTGCTATAGATGCTCCATC 55.0 50.0 58.1 55.6 276-286 bp 1.0 65.9