Hotspot

ycf2

Genome · 899-6547 · 5649 bp

Back to Roya

Pi 0.000531
MarkerSeek score 52.80
Primer available yes
Label ycf2

Feature Metadata

label_name
ycf2
feature_type
gene
region
Genome
length_bp
5649
variable_sites
3
pi
0.000531
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2_p1 ACATTTCATGCCAAAAATGCA AAATTGGATATATGCTTATTCTCA 33.3 25.0 55.8 52.1 291 bp 1.0 40.3
ycf2_p2 CATTTCATGCCAAAAATGCA AAATTGGATATATGCTTATTCTCA 35.0 25.0 53.9 52.1 290 bp 1.0 40.3
ycf2_p3 ACATTTCATGCCAAAAATGC AAATTGGATATATGCTTATTCTCA 35.0 25.0 53.9 52.1 291 bp 1.0 40.3
ycf2_p4 TGCCAAAAATGCATAGCTTA AAATTGGATATATGCTTATTCTCA 35.0 25.0 53.8 52.1 283 bp 1.0 40.3
ycf2_p5 TACATTTCATGCCAAAAATGCA AAATTGGATATATGCTTATTCTCA 31.8 25.0 55.8 52.1 292 bp 1.0 40.3