Hotspot

accD

Genome · 19951-20841 · 891 bp

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Pi 0.00112
MarkerSeek score 59.40
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
Genome
length_bp
891
variable_sites
1
pi
0.00112
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 AAGATTCTTATGTAAAGGGGG CTAGCTCCTAAGGGGCTAGT 38.1 55.0 52.5 57.3 1004 bp 1.0 40.1
accD_p2 AAAGATTCTTATGTAAAGGGGG CTAGCTCCTAAGGGGCTAGT 36.4 55.0 53.5 57.3 1005 bp 1.0 40.1
accD_p3 AAGATTCTTATGTAAAGGGGG AACTAGCTCCTAAGGGGCTA 38.1 50.0 52.5 56.8 1006 bp 1.0 40.1
accD_p4 AAGATTCTTATGTAAAGGGGG TAACTAGCTCCTAAGGGGCT 38.1 50.0 52.5 56.8 1007 bp 1.0 40.1
accD_p5 AAAGATTCTTATGTAAAGGGGG AACTAGCTCCTAAGGGGCTA 36.4 50.0 53.5 56.8 1007 bp 1.0 40.1