Hotspot

orf230-rrn4.5

Genome · 128488-129160 · 673 bp

Back to Roya

Pi 0.00297
MarkerSeek score 60.50
Primer available yes
Label orf230-rrn4.5

Feature Metadata

label_name
orf230-rrn4.5
feature_type
igs
region
Genome
length_bp
673
variable_sites
2
pi
0.00297
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
orf230-rrn4.5_p1 TCGAGAAGGAGGCCGATATT CTCCTAAAAGAGGTCACGGC 50.0 55.0 58.0 58.0 841 bp 1.0 80.1
orf230-rrn4.5_p2 GTTGGTCCCATGATAGGCTG CTCCTAAAAGAGGTCACGGC 55.0 55.0 58.0 58.0 877 bp 1.0 79.9
orf230-rrn4.5_p3 CGAGAAGGAGGCCGATATTG CTCCTAAAAGAGGTCACGGC 55.0 55.0 57.9 58.0 840 bp 1.0 79.5
orf230-rrn4.5_p4 AGTTGGTCCCATGATAGGCT CTCCTAAAAGAGGTCACGGC 50.0 55.0 58.1 58.0 878 bp 1.0 79.3
orf230-rrn4.5_p5 TCGAGAAGGAGGCCGATATT CCTAAAAGAGGTCACGGCAA 50.0 50.0 58.0 57.8 839 bp 1.0 78.7