Hotspot

ndhH-rps15

Genome · 115322-115530 · 209 bp

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Pi 0.0144
MarkerSeek score 60.80
Primer available yes
Label ndhH-rps15

Feature Metadata

label_name
ndhH-rps15
feature_type
igs
region
Genome
length_bp
209
variable_sites
3
pi
0.0144
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH-rps15_p1 CTCCGTGCATTGATGGATGA ATCGTCGAAATCCTATGCAA 50.0 40.0 58.0 55.0 365 bp 1.0 56.0
ndhH-rps15_p2 CTCCGTGCATTGATGGATGA TCGTCGAAATCCTATGCAAT 50.0 40.0 58.0 55.0 364 bp 1.0 56.0
ndhH-rps15_p3 GATGATGTGGTCCCATGCTT ATCGTCGAAATCCTATGCAA 50.0 40.0 57.9 55.0 350 bp 1.0 55.8
ndhH-rps15_p4 GATGATGTGGTCCCATGCTT TCGTCGAAATCCTATGCAAT 50.0 40.0 57.9 55.0 349 bp 1.0 55.8
ndhH-rps15_p5 ACTCCGTGCATTGATGGATG TCGTCGAAATCCTATGCAAT 50.0 40.0 58.3 55.0 365 bp 1.0 53.7