Hotspot

rrn4.5-orf230

Genome · 102042-102714 · 673 bp

Back to Roya

Pi 0.00297
MarkerSeek score 60.50
Primer available yes
Label rrn4.5-orf230

Feature Metadata

label_name
rrn4.5-orf230
feature_type
igs
region
Genome
length_bp
673
variable_sites
2
pi
0.00297
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn4.5-orf230_p1 CTCCTAAAAGAGGTCACGGC TCGAGAAGGAGGCCGATATT 55.0 50.0 58.0 58.0 841 bp 1.0 80.1
rrn4.5-orf230_p2 CTCCTAAAAGAGGTCACGGC GTTGGTCCCATGATAGGCTG 55.0 55.0 58.0 58.0 877 bp 1.0 79.9
rrn4.5-orf230_p3 CTCCTAAAAGAGGTCACGGC CGAGAAGGAGGCCGATATTG 55.0 55.0 58.0 57.9 840 bp 1.0 79.5
rrn4.5-orf230_p4 CTCCTAAAAGAGGTCACGGC AGTTGGTCCCATGATAGGCT 55.0 50.0 58.0 58.1 878 bp 1.0 79.3
rrn4.5-orf230_p5 CCTAAAAGAGGTCACGGCAA TCGAGAAGGAGGCCGATATT 50.0 50.0 57.8 58.0 839 bp 1.0 78.7