Hotspot

trnS-GCU-ycf3

Genome · 81680-82088 · 409 bp

Back to Roya

Pi 0.00489
MarkerSeek score 57.60
Primer available yes
Label trnS-GCU-ycf3

Feature Metadata

label_name
trnS-GCU-ycf3
feature_type
igs
region
Genome
length_bp
409
variable_sites
2
pi
0.00489
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-ycf3_p1 CCTCGGTAAACAAACGCCTA TCATTCCTTTGAGATCTTGGCA 50.0 40.9 57.9 57.7 498 bp 1.0 61.2
trnS-GCU-ycf3_p2 TCGGAAAGAGAGGGATTCGA TCATTCCTTTGAGATCTTGGCA 50.0 40.9 57.9 57.7 520 bp 1.0 60.7
trnS-GCU-ycf3_p3 TCGGTAAACAAACGCCTACA TCATTCCTTTGAGATCTTGGCA 45.0 40.9 57.5 57.7 496 bp 1.0 57.7
trnS-GCU-ycf3_p4 CCTCGGTAAACAAACGCCTA CATTCCTTTGAGATCTTGGCA 50.0 42.9 57.9 56.2 497 bp 1.0 57.4
trnS-GCU-ycf3_p5 TCGGAAAGAGAGGGATTCGA CATTCCTTTGAGATCTTGGCA 50.0 42.9 57.9 56.2 519 bp 1.0 56.9