Hotspot

ycf12-trnY-GUA

Genome · 77384-77578 · 195 bp

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Pi 0.00513
MarkerSeek score 48.30
Primer available yes
Label ycf12-trnY-GUA

Feature Metadata

label_name
ycf12-trnY-GUA
feature_type
igs
region
Genome
length_bp
195
variable_sites
1
pi
0.00513
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf12-trnY-GUA_p1 GTTCTTTCAGGTCCGTTGGT TTGCCTACGCTGGTTCAAAT 50.0 45.0 58.0 58.1 285 bp 1.0 79.2
ycf12-trnY-GUA_p2 GTTCTTTCAGGTCCGTTGGT CCTACGCTGGTTCAAATCCA 50.0 50.0 58.0 57.9 282 bp 1.0 79.1
ycf12-trnY-GUA_p3 GTTCTTTCAGGTCCGTTGGT GGACTGTAAATCCGTTGGCT 50.0 50.0 58.0 57.9 305 bp 1.0 79.1
ycf12-trnY-GUA_p4 AGGTCCGTTGGTAATTGCTC TTGCCTACGCTGGTTCAAAT 50.0 45.0 57.9 58.1 277 bp 1.0 78.6
ycf12-trnY-GUA_p5 GGTCCGTTGGTAATTGCTCT TTGCCTACGCTGGTTCAAAT 50.0 45.0 57.9 58.1 276 bp 1.0 78.6