Hotspot

trnQ-UUG-psbK

Genome · 75666-75883 · 218 bp

Back to Roya

Pi 0
MarkerSeek score 33.80
Primer available yes
Label trnQ-UUG-psbK

Feature Metadata

label_name
trnQ-UUG-psbK
feature_type
igs
region
Genome
length_bp
218
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
33.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-psbK_p1 CGAACCTCCGAATAACAGGG GCTTCAGGCAGCTTAGCTAA 55.0 50.0 58.1 58.0 306 bp 1.0 79.2
trnQ-UUG-psbK_p2 CGAACCTCCGAATAACAGGG AGCTGCTTGCCAAACAAAAG 55.0 45.0 58.1 58.1 391 bp 1.0 79.0
trnQ-UUG-psbK_p3 CGAACCTCCGAATAACAGGG CTGCTTGCCAAACAAAAGCT 55.0 45.0 58.1 58.1 389 bp 1.0 79.0
trnQ-UUG-psbK_p4 CTCCGAATAACAGGGCCAAA GCTTCAGGCAGCTTAGCTAA 50.0 50.0 57.9 58.0 301 bp 1.0 78.8
trnQ-UUG-psbK_p5 CTCCGAATAACAGGGCCAAA CTGCTTGCCAAACAAAAGCT 50.0 45.0 57.9 58.1 384 bp 1.0 78.6