Hotspot

rps7-ndhB

Genome · 55239-55903 · 665 bp

Back to Roya

Pi 0.00752
MarkerSeek score 63.10
Primer available yes
Label rps7-ndhB

Feature Metadata

label_name
rps7-ndhB
feature_type
igs
region
Genome
length_bp
665
variable_sites
5
pi
0.00752
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps7-ndhB_p1 TCGTCAAACAGGGAATGCAA AGTGACTACATTTTGCAAAGCA 45.0 36.4 58.0 57.1 792 bp 1.0 57.0
rps7-ndhB_p2 AATGGATGCTGCTCGTCAAA AGTGACTACATTTTGCAAAGCA 45.0 36.4 58.2 57.1 804 bp 1.0 55.8
rps7-ndhB_p3 TCGTATGGCAGAAGCAAACA AGTGACTACATTTTGCAAAGCA 45.0 36.4 57.8 57.1 750 bp 1.0 55.8
rps7-ndhB_p4 CTGCTCGTCAAACAGGGAAT AGTGACTACATTTTGCAAAGCA 50.0 36.4 58.2 57.1 796 bp 1.0 55.7
rps7-ndhB_p5 CTCATCGTATGGCAGAAGCA AGTGACTACATTTTGCAAAGCA 50.0 36.4 57.8 57.1 754 bp 1.0 55.4