Hotspot

rpl16-rps3

Genome · 48008-49086 · 1079 bp

Back to Roya

Pi 0.000927
MarkerSeek score 59.30
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
Genome
length_bp
1079
variable_sites
1
pi
0.000927
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 CCGTGTTGCTATTCCCTTCA CAAACTTTGCGTGCACAGAT 50.0 45.0 57.9 57.9 1253-1257 bp 1.0 78.2
rpl16-rps3_p2 CTTGAAGCCCGAATCTTCCA CAAACTTTGCGTGCACAGAT 50.0 45.0 57.9 57.9 1290-1294 bp 1.0 78.2
rpl16-rps3_p3 TTTCCCCGTGTTGCTATTCC CAAACTTTGCGTGCACAGAT 50.0 45.0 58.2 57.9 1258-1262 bp 1.0 77.7
rpl16-rps3_p4 CCGTGTTGCTATTCCCTTCA CGCGTACCTCTCCAAACTTT 50.0 50.0 57.9 58.2 1265-1269 bp 1.0 77.5
rpl16-rps3_p5 CTTGAAGCCCGAATCTTCCA CGCGTACCTCTCCAAACTTT 50.0 50.0 57.9 58.2 1302-1306 bp 1.0 77.5