Hotspot

psbA-psbC

Genome · 25902-26814 · 913 bp

Back to Roya

Pi 0.00219
MarkerSeek score 60.10
Primer available yes
Label psbA-psbC

Feature Metadata

label_name
psbA-psbC
feature_type
igs
region
Genome
length_bp
913
variable_sites
2
pi
0.00219
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-psbC_p1 CACTTGGGCTGACATCATCA CATTTGTGGCATGCAGGAAG 50.0 50.0 57.9 58.0 1132-1133 bp 1.0 79.2
psbA-psbC_p2 ACACTTGGGCTGACATCATC CATTTGTGGCATGCAGGAAG 50.0 50.0 57.9 58.0 1133-1134 bp 1.0 79.2
psbA-psbC_p3 TTGGGCTGACATCATCAACC CATTTGTGGCATGCAGGAAG 50.0 50.0 58.2 58.0 1129-1130 bp 1.0 78.9
psbA-psbC_p4 CACTTGGGCTGACATCATCA ATTTGTGGCATGCAGGAAGA 50.0 45.0 57.9 58.1 1131-1132 bp 1.0 78.7
psbA-psbC_p5 ACACTTGGGCTGACATCATC ATTTGTGGCATGCAGGAAGA 50.0 45.0 57.9 58.1 1132-1133 bp 1.0 78.7