Hotspot

trnT-GGU

LSC · 16325-16389 · 65 bp

Back to Rottboellia

Pi 0.0615

MarkerSeek score 73.20

Primer available yes

Label trnT-GGU

Feature Metadata

label_name: trnT-GGU
feature_type: tRNA
region: LSC
length_bp: 65
variable_sites: 4
pi: 0.0615
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU_p1	AGGCATAAGTCATCGGTTCA	TCTCGCACTAAACTAAGTCA	45.0	40.0	56.6	53.8	185-187 bp	1.0	41.7
trnT-GGU_p2	TGGTAAGGCATAAGTCATCGG	TCTCGCACTAAACTAAGTCA	47.6	40.0	57.5	53.8	190-192 bp	1.0	41.7
trnT-GGU_p3	GGTAAGGCATAAGTCATCGGT	TCTCGCACTAAACTAAGTCA	47.6	40.0	57.5	53.8	189-191 bp	1.0	41.7
trnT-GGU_p4	GTGGTAGAGTAATGCCATGGT	TCTCGCACTAAACTAAGTCA	47.6	40.0	57.4	53.8	207-209 bp	1.0	41.5
trnT-GGU_p5	AGCCCTTTTAACTCAGTGGT	TCTCGCACTAAACTAAGTCA	45.0	40.0	56.7	53.8	222-224 bp	1.0	41.4