Hotspot

ndhA

SSC · 114303-116410 · 2108 bp

Back to Rottboellia

Pi 0.00954

MarkerSeek score 49.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2108
variable_sites: 20
pi: 0.00954
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	AAGGGGTAGATCTATCGGGC	GGCTTATCGGGACCAATGTT	55.0	50.0	58.1	57.9	2980-2991 bp	1.0	80.6
ndhA_p2	AAGGGGTAGATCTATCGGGC	AAATTGGGGCTTATCGGGAC	55.0	50.0	58.1	57.9	2987-2998 bp	1.0	80.5
ndhA_p3	AAGGGGTAGATCTATCGGGC	GTAGAAGCTCCAAAAGGCGA	55.0	50.0	58.1	57.9	2644-2655 bp	1.0	80.3
ndhA_p4	AAGGGGTAGATCTATCGGGC	AGTAGAAGCTCCAAAAGGCG	55.0	50.0	58.1	57.9	2645-2656 bp	1.0	80.2
ndhA_p5	AAGGGGTAGATCTATCGGGC	GGGACCAATGTTACGAGCTT	55.0	50.0	58.1	57.9	2972-2983 bp	1.0	80.1