Hotspot

ndhI-ndhA

SSC · 114192-114302 · 111 bp

Back to Rottboellia

Pi 0.131

MarkerSeek score 87.00

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 111
variable_sites: 14
pi: 0.131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 87.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	TGACCAATGTATCTTGCGGC	TGGACTTTACCCAGGATGAGA	50.0	47.6	58.6	57.8	295-299 bp	1.0	70.3
ndhI-ndhA_p2	TGACCAATGTATCTTGCGGC	ACCCAGGATGAGAATGGATCA	50.0	47.6	58.6	58.2	287-291 bp	1.0	70.3
ndhI-ndhA_p3	CGCGTATTGTTTGTTGACCA	TGGACTTTACCCAGGATGAGA	45.0	47.6	57.3	57.8	276-280 bp	1.0	70.2
ndhI-ndhA_p4	GCGCGTATTGTTTGTTGACC	TGGACTTTACCCAGGATGAGA	50.0	47.6	58.7	57.8	277-281 bp	1.0	70.2
ndhI-ndhA_p5	GGCGCGTATTGTTTGTTGAC	TGGACTTTACCCAGGATGAGA	50.0	47.6	58.7	57.8	278-282 bp	1.0	70.1