Hotspot

rpl32-trnL-UAG

SSC · 108256-108774 · 519 bp

Back to Rottboellia

Pi 0.0312

MarkerSeek score 62.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 519
variable_sites: 16
pi: 0.0312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGAAGGATTGATTTGCGGCA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.2	58.0	832-843 bp	1.0	81.3
rpl32-trnL-UAG_p2	AGAAGGATTGATTTGCGGCA	GGATTCGAACCGAGATGCTT	45.0	50.0	58.2	58.1	835-846 bp	1.0	80.8
rpl32-trnL-UAG_p3	AGAAGGATTGATTTGCGGCA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.2	57.9	805-816 bp	1.0	80.4
rpl32-trnL-UAG_p4	AGAAGGATTGATTTGCGGCA	GATCCGCACAAAAGAGTTGC	45.0	50.0	58.2	58.0	1384-1395 bp	1.0	80.2
rpl32-trnL-UAG_p5	AGAAGGATTGATTTGCGGCA	ATGTCCCGAAGAAACCCAAC	45.0	50.0	58.2	58.1	1118-1129 bp	1.0	80.0