Hotspot

rpl22-rps19

LSC · 82242-82333 · 92 bp

Back to Rottboellia

Pi 0.0326

MarkerSeek score 71.20

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 92
variable_sites: 3
pi: 0.0326
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CGGTTGGAACTTTTCTTGCG	CAAATTGGGGGAATTCGTGC	50.0	50.0	58.2	58.0	253-256 bp	1.0	79.8
rpl22-rps19_p2	CGGTTGGAACTTTTCTTGCG	GCAAATTGGGGGAATTCGTG	50.0	50.0	58.2	58.0	254-257 bp	1.0	79.8
rpl22-rps19_p3	CGGTTGGAACTTTTCTTGCG	ATGGTAGGTCGCAAATTGGG	50.0	50.0	58.2	58.2	264-267 bp	1.0	77.9
rpl22-rps19_p4	TCTCAGCCCCTTCTTCTTCT	CAAATTGGGGGAATTCGTGC	50.0	50.0	57.7	58.0	231 bp	0.5	57.3
rpl22-rps19_p5	TCTCAGCCCCTTCTTCTTCT	GCAAATTGGGGGAATTCGTG	50.0	50.0	57.7	58.0	232 bp	0.5	57.3