Hotspot

rpoA-rps11

LSC · 77078-77149 · 72 bp

Back to Rottboellia

Pi 0.0303

MarkerSeek score 67.90

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 72
variable_sites: 2
pi: 0.0303
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	AGCGGAGACAGAATGAAACG	GATGTAACACCTATGCCGCA	50.0	50.0	58.3	58.1	233-239 bp	1.0	80.0
rpoA-rps11_p2	GCGGAGACAGAATGAAACGA	GATGTAACACCTATGCCGCA	50.0	50.0	58.3	58.1	232-238 bp	1.0	80.0
rpoA-rps11_p3	AGCGGAGACAGAATGAAACG	ACACCTATGCCGCATAATGG	50.0	50.0	58.3	58.1	227-233 bp	1.0	79.7
rpoA-rps11_p4	GCGGAGACAGAATGAAACGA	ACACCTATGCCGCATAATGG	50.0	50.0	58.3	58.1	226-232 bp	1.0	79.6
rpoA-rps11_p5	AGCGGAGACAGAATGAAACG	CGCATAATGGATGTCGACCT	50.0	50.0	58.3	57.8	217-223 bp	1.0	79.4