Hotspot

rpl20-rps12

LSC · 68493-69171 · 679 bp

Back to Rottboellia

Pi 0.0177

MarkerSeek score 67.30

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 679
variable_sites: 12
pi: 0.0177
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	ACGCATTTTTGTTCGTCGTC	GAGGAACATGTGCTAGGGTG	45.0	55.0	58.0	58.0	756-761 bp	1.0	80.9
rpl20-rps12_p2	ACGCATTTTTGTTCGTCGTC	CGACATGCCAACGGTTAAAC	45.0	50.0	58.0	58.0	851-856 bp	1.0	80.7
rpl20-rps12_p3	ACGCATTTTTGTTCGTCGTC	GTCGAGGAACATGTGCTAGG	45.0	55.0	58.0	58.1	759-764 bp	1.0	80.6
rpl20-rps12_p4	GTTTGAGGCAAATGAACGCA	GAGGAACATGTGCTAGGGTG	45.0	55.0	57.9	58.0	771-776 bp	1.0	79.9
rpl20-rps12_p5	GTTTGAGGCAAATGAACGCA	CGACATGCCAACGGTTAAAC	45.0	50.0	57.9	58.0	866-871 bp	1.0	79.7