Hotspot

rbcL-psaI

LSC · 58190-59055 · 866 bp

Back to Rottboellia

Pi 0.014

MarkerSeek score 67.40

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 866
variable_sites: 12
pi: 0.014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	TGCAAATGGAGTGCTGAACT	GTCATTGCAATTGCCGGAAA	45.0	45.0	58.0	57.9	1024-1144 bp	1.0	80.6
rbcL-psaI_p2	GCCGCAGCTTGTGAAATATG	GTCATTGCAATTGCCGGAAA	50.0	45.0	58.2	57.9	1003-1123 bp	1.0	79.4
rbcL-psaI_p3	TGCAAATGGAGTGCTGAACT	GAAGTCATTGCAATTGCCGG	45.0	50.0	58.0	58.4	1027-1147 bp	1.0	78.3
rbcL-psaI_p4	TGCAAATGGAGTGCTGAACT	GGAAGTCATTGCAATTGCCG	45.0	50.0	58.0	58.4	1028-1148 bp	1.0	78.3
rbcL-psaI_p5	GCCGCAGCTTGTGAAATATG	GAAGTCATTGCAATTGCCGG	50.0	50.0	58.2	58.4	1006-1126 bp	1.0	77.1