Hotspot

trnR-UCU-trnfM-CAU

LSC · 38097-38262 · 166 bp

Back to Rottboellia

Pi 0.0482

MarkerSeek score 76.50

Primer available yes

Label trnR-UCU-trnfM-CAU

Feature Metadata

label_name: trnR-UCU-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 166
variable_sites: 8
pi: 0.0482
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnfM-CAU_p1	AGAAGACCTCTGTCCTATCCA	TCCCCGCTAAGAAGGTAGAG	47.6	55.0	56.9	57.9	270-277 bp	1.0	65.4
trnR-UCU-trnfM-CAU_p2	AGGTTTAGAAGACCTCTGTCCT	TCCCCGCTAAGAAGGTAGAG	45.5	55.0	57.9	57.9	276-283 bp	1.0	64.7
trnR-UCU-trnfM-CAU_p3	AGAAGACCTCTGTCCTATCCA	CCAACCCCCTTTTATCCACC	47.6	55.0	56.9	58.1	293-300 bp	1.0	64.6
trnR-UCU-trnfM-CAU_p4	AGGTTTAGAAGACCTCTGTCCT	CCAACCCCCTTTTATCCACC	45.5	55.0	57.9	58.1	299-306 bp	1.0	63.9
trnR-UCU-trnfM-CAU_p5	AGAAGACCTCTGTCCTATCCA	ACCCCCTTTTATCCACCAGT	47.6	50.0	56.9	58.2	290-297 bp	1.0	63.9