Hotspot

petN-trnC-GCA

LSC · 19554-20216 · 663 bp

Back to Rottboellia

Pi 0.0217

MarkerSeek score 62.50

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 663
variable_sites: 13
pi: 0.0217
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	CTGCAAATCCTTTACCCCCA	50.0	50.0	58.2	57.8	731-940 bp	1.0	82.2
petN-trnC-GCA_p2	AGCCCAAGCGAGACTTACTA	TGCAAATCCTTTACCCCCAG	50.0	50.0	58.2	57.8	730-939 bp	1.0	82.2
petN-trnC-GCA_p3	AGCCCAAGCGAGACTTACTA	ACTGCAAATCCTTTACCCCC	50.0	50.0	58.2	57.8	732-941 bp	1.0	82.2
petN-trnC-GCA_p4	AGCCCAAGCGAGACTTACTA	GCAAATCCTTTACCCCCAGT	50.0	50.0	58.2	57.8	729-938 bp	1.0	82.2
petN-trnC-GCA_p5	GCCCAAGCGAGACTTACTAT	TGCAAATCCTTTACCCCCAG	50.0	50.0	57.1	57.8	729-938 bp	1.0	76.3