Hotspot

trnG-GCC-trnfM-CAU

LSC · 12890-13379 · 490 bp

Back to Rottboellia

Pi 0.0661

MarkerSeek score 76.80

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 490
variable_sites: 25
pi: 0.0661
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	AGGAGAAGATACGGGTTCGA	CGAGGCTCATAACCTTGAGG	50.0	55.0	57.6	57.8	460-572 bp	1.0	82.6
trnG-GCC-trnfM-CAU_p2	GTAGCGAGCGTAGTTCAGTG	CGAGGCTCATAACCTTGAGG	55.0	55.0	58.5	57.8	499-611 bp	1.0	82.0
trnG-GCC-trnfM-CAU_p3	AGGAGAAGATACGGGTTCGA	CAGTTTGGTAGCTCACGAGG	50.0	55.0	57.6	58.3	475-587 bp	1.0	82.0
trnG-GCC-trnfM-CAU_p4	AGGAGAAGATACGGGTTCGA	CAGTTTTCGGCCCTACAGAG	50.0	55.0	57.6	58.3	505-617 bp	1.0	81.9
trnG-GCC-trnfM-CAU_p5	ATGTAGCGAGCGTAGTTCAG	CGAGGCTCATAACCTTGAGG	50.0	55.0	57.5	57.8	501-613 bp	1.0	81.9