primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	trnG-GCC-trnfM-CAU	1	AGGAGAAGATACGGGTTCGA	CGAGGCTCATAACCTTGAGG	20	20	50.0	55.0	57.6	57.8	0.0	16.0	0.663168	12890	13379	460-572	1.000000	82.6
trnG-GCC-trnfM-CAU_p2	trnG-GCC-trnfM-CAU	2	GTAGCGAGCGTAGTTCAGTG	CGAGGCTCATAACCTTGAGG	20	20	55.0	55.0	58.5	57.8	0.0	16.0	0.695065	12890	13379	499-611	1.000000	82.0
trnG-GCC-trnfM-CAU_p3	trnG-GCC-trnfM-CAU	3	AGGAGAAGATACGGGTTCGA	CAGTTTGGTAGCTCACGAGG	20	20	50.0	55.0	57.6	58.3	0.0	0.0	0.717159	12890	13379	475-587	1.000000	82.0
trnG-GCC-trnfM-CAU_p4	trnG-GCC-trnfM-CAU	4	AGGAGAAGATACGGGTTCGA	CAGTTTTCGGCCCTACAGAG	20	20	50.0	55.0	57.6	58.3	0.0	0.0	0.705100	12890	13379	505-617	1.000000	81.9
trnG-GCC-trnfM-CAU_p5	trnG-GCC-trnfM-CAU	5	ATGTAGCGAGCGTAGTTCAG	CGAGGCTCATAACCTTGAGG	20	20	50.0	55.0	57.5	57.8	0.0	16.0	0.708087	12890	13379	501-613	1.000000	81.9
trnG-UCC-trnM-CAU_p1	trnG-UCC-trnM-CAU	1	ACGAATCACACTTTTACCACT	ACCACTAAGTGAAAAGCCCT	21	20	38.1	45.0	55.5	56.7	0.0	0.0	4.884212	14312	16260	2063-2076	1.000000	43.4
trnG-UCC-trnM-CAU_p2	trnG-UCC-trnM-CAU	2	ACGAATCACACTTTTACCACT	TGAACCGATGACTTATGCCT	21	20	38.1	45.0	55.5	56.6	0.0	0.0	4.907539	14312	16260	2033-2046	1.000000	43.3
trnG-UCC-trnM-CAU_p3	trnG-UCC-trnM-CAU	3	ACGAATCACACTTTTACCACT	GACTTCTGTCTTACCAAGGCA	21	21	38.1	47.6	55.5	57.6	0.0	0.0	4.938470	14312	16260	2091-2104	1.000000	43.0
trnG-UCC-trnM-CAU_p4	trnG-UCC-trnM-CAU	4	ACGAATCACACTTTTACCACT	TGACTTCTGTCTTACCAAGGC	21	21	38.1	47.6	55.5	57.6	0.0	0.0	4.938470	14312	16260	2092-2105	1.000000	43.0
trnG-UCC-trnM-CAU_p5	trnG-UCC-trnM-CAU	5	ACGAATCACACTTTTACCACT	CCGATGACTTATGCCTTACCA	21	21	38.1	47.6	55.5	57.5	0.0	0.0	5.004851	14312	16260	2029-2042	1.000000	42.6
trnT-GGU_p1	trnT-GGU	1	AGGCATAAGTCATCGGTTCA	TCTCGCACTAAACTAAGTCA	20	20	45.0	40.0	56.6	53.8	0.0	0.0	5.578010	16325	16389	185-187	1.000000	41.7
trnT-GGU_p2	trnT-GGU	2	TGGTAAGGCATAAGTCATCGG	TCTCGCACTAAACTAAGTCA	21	20	47.6	40.0	57.5	53.8	0.0	0.0	5.675322	16325	16389	190-192	1.000000	41.7
trnT-GGU_p3	trnT-GGU	3	GGTAAGGCATAAGTCATCGGT	TCTCGCACTAAACTAAGTCA	21	20	47.6	40.0	57.5	53.8	0.0	0.0	5.676958	16325	16389	189-191	1.000000	41.7
trnT-GGU_p4	trnT-GGU	4	GTGGTAGAGTAATGCCATGGT	TCTCGCACTAAACTAAGTCA	21	20	47.6	40.0	57.4	53.8	14.7	0.0	5.766950	16325	16389	207-209	1.000000	41.5
trnT-GGU_p5	trnT-GGU	5	AGCCCTTTTAACTCAGTGGT	TCTCGCACTAAACTAAGTCA	20	20	45.0	40.0	56.7	53.8	0.0	0.0	5.554683	16325	16389	222-224	1.000000	41.4
trnY-GUA-trnD-GUC_p1	trnY-GUA-trnD-GUC	1	AATTCTTCCTGGGTCGATGC	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	58.0	58.5	0.0	7.1	0.521454	17078	17426	473-473	1.000000	77.0
trnY-GUA-trnD-GUC_p2	trnY-GUA-trnD-GUC	2	GGGGACGGACTGTAAATTCG	CGGTGCTCTGACCAATTGAA	20	20	55.0	50.0	58.4	58.5	0.0	7.1	0.828828	17078	17426	440-440	1.000000	74.6
trnY-GUA-trnD-GUC_p3	trnY-GUA-trnD-GUC	3	GGGACGGACTGTAAATTCGT	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	57.6	58.5	0.0	7.1	0.852505	17078	17426	439-439	1.000000	74.5
trnY-GUA-trnD-GUC_p4	trnY-GUA-trnD-GUC	4	AATTCTTCCTGGGTCGATGC	GGTGCTCTGACCAATTGAAC	20	20	50.0	50.0	58.0	57.0	0.0	7.1	1.041608	17078	17426	472-472	1.000000	72.9
trnY-GUA-trnD-GUC_p5	trnY-GUA-trnD-GUC	5	TGGGGACGGACTGTAAATTC	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	57.2	58.5	0.0	7.1	1.251324	17078	17426	441-441	1.000000	71.3
petN-trnC-GCA_p1	petN-trnC-GCA	1	AGCCCAAGCGAGACTTACTA	CTGCAAATCCTTTACCCCCA	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.381478	19554	20216	731-940	1.000000	82.2
petN-trnC-GCA_p2	petN-trnC-GCA	2	AGCCCAAGCGAGACTTACTA	TGCAAATCCTTTACCCCCAG	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.381478	19554	20216	730-939	1.000000	82.2
petN-trnC-GCA_p3	petN-trnC-GCA	3	AGCCCAAGCGAGACTTACTA	ACTGCAAATCCTTTACCCCC	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.383064	19554	20216	732-941	1.000000	82.2
petN-trnC-GCA_p4	petN-trnC-GCA	4	AGCCCAAGCGAGACTTACTA	GCAAATCCTTTACCCCCAGT	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.383064	19554	20216	729-938	1.000000	82.2
petN-trnC-GCA_p5	petN-trnC-GCA	5	GCCCAAGCGAGACTTACTAT	TGCAAATCCTTTACCCCCAG	20	20	50.0	50.0	57.1	57.8	0.0	0.0	1.122914	19554	20216	729-938	1.000000	76.3
trnR-UCU-trnfM-CAU_p1	trnR-UCU-trnfM-CAU	1	AGAAGACCTCTGTCCTATCCA	TCCCCGCTAAGAAGGTAGAG	21	20	47.6	55.0	56.9	57.9	0.0	0.0	2.120439	38097	38262	270-277	1.000000	65.4
trnR-UCU-trnfM-CAU_p2	trnR-UCU-trnfM-CAU	2	AGGTTTAGAAGACCTCTGTCCT	TCCCCGCTAAGAAGGTAGAG	22	20	45.5	55.0	57.9	57.9	0.0	0.0	2.203767	38097	38262	276-283	1.000000	64.7
trnR-UCU-trnfM-CAU_p3	trnR-UCU-trnfM-CAU	3	AGAAGACCTCTGTCCTATCCA	CCAACCCCCTTTTATCCACC	21	20	47.6	55.0	56.9	58.1	0.0	0.0	2.190963	38097	38262	293-300	1.000000	64.6
trnR-UCU-trnfM-CAU_p4	trnR-UCU-trnfM-CAU	4	AGGTTTAGAAGACCTCTGTCCT	CCAACCCCCTTTTATCCACC	22	20	45.5	55.0	57.9	58.1	0.0	0.0	2.274291	38097	38262	299-306	1.000000	63.9
trnR-UCU-trnfM-CAU_p5	trnR-UCU-trnfM-CAU	5	AGAAGACCTCTGTCCTATCCA	ACCCCCTTTTATCCACCAGT	21	20	47.6	50.0	56.9	58.2	0.0	0.0	2.283339	38097	38262	290-297	1.000000	63.9
ycf3_p1	ycf3	1	TCTTCAACCAAACCACCCTT	TCTATATAGAATGGGTTGGGA	20	21	45.0	38.1	57.2	52.5	0.0	10.1	7.347617	44021	46035	2047-2081	1.000000	43.1
ycf3_p2	ycf3	2	TCTTCAACCAAACCACCCTT	TCTATATAGAATGGGTTGGGAA	20	22	45.0	36.4	57.2	53.4	0.0	10.1	7.420392	44021	46035	2047-2081	1.000000	43.1
ycf3_p3	ycf3	3	TCTTCAACCAAACCACCCTT	TTCTATATAGAATGGGTTGGGA	20	22	45.0	36.4	57.2	53.4	0.0	19.2	7.420392	44021	46035	2048-2082	1.000000	43.1
ycf3_p4	ycf3	4	TCTTCAACCAAACCACCCTT	TTCTATATAGAATGGGTTGGGAA	20	23	45.0	34.8	57.2	54.3	0.0	19.2	7.575905	44021	46035	2048-2082	1.000000	43.1
ycf3_p5	ycf3	5	ATCTTCAACCAAACCACCCT	TCTATATAGAATGGGTTGGGA	20	21	45.0	38.1	56.9	52.5	0.0	10.1	7.581353	44021	46035	2048-2082	1.000000	43.1
trnV-UAC-trnM-CAU_p1	trnV-UAC-trnM-CAU	1	AACGAGTTGCTCTACCGAAC	CCCGCCGTATGAAAGCAATA	20	20	50.0	50.0	57.9	58.4	0.0	0.0	0.473066	53633	53862	308-314	1.000000	80.1
trnV-UAC-trnM-CAU_p2	trnV-UAC-trnM-CAU	2	AACGAGTTGCTCTACCGAAC	CCGCCGTATGAAAGCAATAC	20	20	50.0	50.0	57.9	57.3	0.0	0.0	0.725019	53633	53862	307-313	1.000000	78.1
trnV-UAC-trnM-CAU_p3	trnV-UAC-trnM-CAU	3	CGAGTTGCTCTACCGAACTG	CCCGCCGTATGAAAGCAATA	20	20	55.0	50.0	58.4	58.4	0.0	0.0	0.792465	53633	53862	306-312	1.000000	77.5
trnV-UAC-trnM-CAU_p4	trnV-UAC-trnM-CAU	4	AACGAGTTGCTCTACCGAAC	TTTGAACCAATGACTCCCGC	20	20	50.0	50.0	57.9	58.8	0.0	0.0	0.817838	53633	53862	323-329	1.000000	77.1
trnV-UAC-trnM-CAU_p5	trnV-UAC-trnM-CAU	5	AACGAGTTGCTCTACCGAAC	AGCGAAGTAAGTCCAATGCT	20	20	50.0	45.0	57.9	57.2	0.0	0.0	0.831904	53633	53862	380-386	1.000000	76.5
rbcL-psaI_p1	rbcL-psaI	1	TGCAAATGGAGTGCTGAACT	GTCATTGCAATTGCCGGAAA	20	20	45.0	45.0	58.0	57.9	0.0	26.9	0.091940	58190	59055	1024-1144	1.000000	80.6
rbcL-psaI_p2	rbcL-psaI	2	GCCGCAGCTTGTGAAATATG	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	58.2	57.9	0.0	26.9	0.246070	58190	59055	1003-1123	1.000000	79.4
rbcL-psaI_p3	rbcL-psaI	3	TGCAAATGGAGTGCTGAACT	GAAGTCATTGCAATTGCCGG	20	20	45.0	50.0	58.0	58.4	0.0	26.9	0.382105	58190	59055	1027-1147	1.000000	78.3
rbcL-psaI_p4	rbcL-psaI	4	TGCAAATGGAGTGCTGAACT	GGAAGTCATTGCAATTGCCG	20	20	45.0	50.0	58.0	58.4	0.0	26.9	0.382105	58190	59055	1028-1148	1.000000	78.3
rbcL-psaI_p5	rbcL-psaI	5	GCCGCAGCTTGTGAAATATG	GAAGTCATTGCAATTGCCGG	20	20	50.0	50.0	58.2	58.4	0.0	26.9	0.536235	58190	59055	1006-1126	1.000000	77.1
psbL-psbF_p1	psbL-psbF	1	CATTCGGGTTTGATTGTGTCA	CACGGATTAGCTGTACCCAC	21	20	42.9	55.0	57.1	58.1	0.0	0.0	1.977616	63610	63631	113-113	1.000000	65.6
psbL-psbF_p2	psbL-psbF	2	CATTCGGGTTTGATTGTGTCA	TCACGGATTAGCTGTACCCA	21	20	42.9	50.0	57.1	58.1	0.0	0.0	2.058253	63610	63631	114-114	1.000000	64.9
psbL-psbF_p3	psbL-psbF	3	CATTCGGGTTTGATTGTGTCA	GCTGTTCACGGATTAGCTGT	21	20	42.9	50.0	57.1	58.3	0.0	0.0	2.186695	63610	63631	119-119	1.000000	63.9
psbL-psbF_p4	psbL-psbF	4	TCATTCGGGTTTGATTGTGTC	CACGGATTAGCTGTACCCAC	21	20	42.9	55.0	56.8	58.1	0.0	0.0	2.252004	63610	63631	114-114	1.000000	63.4
psbL-psbF_p5	psbL-psbF	5	TGTTCATTCGGGTTTGATTGTG	CACGGATTAGCTGTACCCAC	22	20	40.9	55.0	57.8	58.1	0.0	0.0	2.300006	63610	63631	117-117	1.000000	63.0
psbE-petL_p1	psbE-petL	1	TGACAAATAACCAACCCGCA	AAGCAGCCAGTAGAAAACCG	20	20	45.0	50.0	58.0	58.5	0.0	0.0	0.493582	64014	65275	1420-1427	1.000000	77.1
psbE-petL_p2	psbE-petL	2	TGACAAATAACCAACCCGCA	GCAGCCAGTAGAAAACCGAA	20	20	45.0	50.0	58.0	58.5	0.0	0.0	0.497931	64014	65275	1418-1425	1.000000	77.1
psbE-petL_p3	psbE-petL	3	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.527577	64014	65275	1409-1416	1.000000	76.9
psbE-petL_p4	psbE-petL	4	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.531926	64014	65275	1407-1414	1.000000	76.8
psbE-petL_p5	psbE-petL	5	ACCAACCCGCAATGAATAGG	AAGCAGCCAGTAGAAAACCG	20	20	50.0	50.0	58.2	58.5	0.0	0.0	0.713535	64014	65275	1411-1418	1.000000	75.4
rpl20-rps12_p1	rpl20-rps12	1	ACGCATTTTTGTTCGTCGTC	GAGGAACATGTGCTAGGGTG	20	20	45.0	55.0	58.0	58.0	0.0	3.2	0.043124	68493	69171	756-761	1.000000	80.9
rpl20-rps12_p2	rpl20-rps12	2	ACGCATTTTTGTTCGTCGTC	CGACATGCCAACGGTTAAAC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.054869	68493	69171	851-856	1.000000	80.7
rpl20-rps12_p3	rpl20-rps12	3	ACGCATTTTTGTTCGTCGTC	GTCGAGGAACATGTGCTAGG	20	20	45.0	55.0	58.0	58.1	0.0	3.2	0.085391	68493	69171	759-764	1.000000	80.6
rpl20-rps12_p4	rpl20-rps12	4	GTTTGAGGCAAATGAACGCA	GAGGAACATGTGCTAGGGTG	20	20	45.0	55.0	57.9	58.0	0.0	3.2	0.162578	68493	69171	771-776	1.000000	79.9
rpl20-rps12_p5	rpl20-rps12	5	GTTTGAGGCAAATGAACGCA	CGACATGCCAACGGTTAAAC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.174323	68493	69171	866-871	1.000000	79.7
rpoA-rps11_p1	rpoA-rps11	1	AGCGGAGACAGAATGAAACG	GATGTAACACCTATGCCGCA	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.337101	77078	77149	233-239	1.000000	80.0
rpoA-rps11_p2	rpoA-rps11	2	GCGGAGACAGAATGAAACGA	GATGTAACACCTATGCCGCA	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.341883	77078	77149	232-238	1.000000	80.0
rpoA-rps11_p3	rpoA-rps11	3	AGCGGAGACAGAATGAAACG	ACACCTATGCCGCATAATGG	20	20	50.0	50.0	58.3	58.1	0.0	20.6	0.388986	77078	77149	227-233	1.000000	79.7
rpoA-rps11_p4	rpoA-rps11	4	GCGGAGACAGAATGAAACGA	ACACCTATGCCGCATAATGG	20	20	50.0	50.0	58.3	58.1	0.0	20.6	0.393767	77078	77149	226-232	1.000000	79.6
rpoA-rps11_p5	rpoA-rps11	5	AGCGGAGACAGAATGAAACG	CGCATAATGGATGTCGACCT	20	20	50.0	50.0	58.3	57.8	0.0	14.1	0.440060	77078	77149	217-223	1.000000	79.4
rpl22-rps19_p1	rpl22-rps19	1	CGGTTGGAACTTTTCTTGCG	CAAATTGGGGGAATTCGTGC	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.254796	82242	82333	253-256	1.000000	79.8
rpl22-rps19_p2	rpl22-rps19	2	CGGTTGGAACTTTTCTTGCG	GCAAATTGGGGGAATTCGTG	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.254796	82242	82333	254-257	1.000000	79.8
rpl22-rps19_p3	rpl22-rps19	3	CGGTTGGAACTTTTCTTGCG	ATGGTAGGTCGCAAATTGGG	20	20	50.0	50.0	58.2	58.2	0.0	3.4	0.483336	82242	82333	264-267	1.000000	77.9
rpl22-rps19_p4	rpl22-rps19	4	TCTCAGCCCCTTCTTCTTCT	CAAATTGGGGGAATTCGTGC	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.342757	82242	82333	231-231	0.500000	57.3
rpl22-rps19_p5	rpl22-rps19	5	TCTCAGCCCCTTCTTCTTCT	GCAAATTGGGGGAATTCGTG	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.342757	82242	82333	232-232	0.500000	57.3
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AGAAGGATTGATTTGCGGCA	TTCGAACCGAGATGCTTGAG	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.165596	108256	108774	832-843	1.000000	81.3
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGAAGGATTGATTTGCGGCA	GGATTCGAACCGAGATGCTT	20	20	45.0	50.0	58.2	58.1	0.0	0.0	0.220563	108256	108774	835-846	1.000000	80.8
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGAAGGATTGATTTGCGGCA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.2	57.9	0.0	0.0	0.286165	108256	108774	805-816	1.000000	80.4
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGAAGGATTGATTTGCGGCA	GATCCGCACAAAAGAGTTGC	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.192785	108256	108774	1384-1395	1.000000	80.2
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGAAGGATTGATTTGCGGCA	ATGTCCCGAAGAAACCCAAC	20	20	45.0	50.0	58.2	58.1	0.0	0.0	0.256250	108256	108774	1118-1129	1.000000	80.0
ndhI-ndhA_p1	ndhI-ndhA	1	TGACCAATGTATCTTGCGGC	TGGACTTTACCCAGGATGAGA	20	21	50.0	47.6	58.6	57.8	0.0	0.0	1.820911	114192	114302	295-299	1.000000	70.3
ndhI-ndhA_p2	ndhI-ndhA	2	TGACCAATGTATCTTGCGGC	ACCCAGGATGAGAATGGATCA	20	21	50.0	47.6	58.6	58.2	0.0	0.0	1.841055	114192	114302	287-291	1.000000	70.3
ndhI-ndhA_p3	ndhI-ndhA	3	CGCGTATTGTTTGTTGACCA	TGGACTTTACCCAGGATGAGA	20	21	45.0	47.6	57.3	57.8	0.0	0.0	1.871629	114192	114302	276-280	1.000000	70.2
ndhI-ndhA_p4	ndhI-ndhA	4	GCGCGTATTGTTTGTTGACC	TGGACTTTACCCAGGATGAGA	20	21	50.0	47.6	58.7	57.8	8.4	0.0	1.875651	114192	114302	277-281	1.000000	70.2
ndhI-ndhA_p5	ndhI-ndhA	5	GGCGCGTATTGTTTGTTGAC	TGGACTTTACCCAGGATGAGA	20	21	50.0	47.6	58.7	57.8	8.4	0.0	1.875651	114192	114302	278-282	1.000000	70.1
ndhA_p1	ndhA	1	AAGGGGTAGATCTATCGGGC	GGCTTATCGGGACCAATGTT	20	20	55.0	50.0	58.1	57.9	5.6	0.0	0.112802	114303	116410	2980-2991	1.000000	80.6
ndhA_p2	ndhA	2	AAGGGGTAGATCTATCGGGC	AAATTGGGGCTTATCGGGAC	20	20	55.0	50.0	58.1	57.9	5.6	0.0	0.127385	114303	116410	2987-2998	1.000000	80.5
ndhA_p3	ndhA	3	AAGGGGTAGATCTATCGGGC	GTAGAAGCTCCAAAAGGCGA	20	20	55.0	50.0	58.1	57.9	5.6	0.0	0.154782	114303	116410	2644-2655	1.000000	80.3
ndhA_p4	ndhA	4	AAGGGGTAGATCTATCGGGC	AGTAGAAGCTCCAAAAGGCG	20	20	55.0	50.0	58.1	57.9	5.6	0.0	0.160625	114303	116410	2645-2656	1.000000	80.2
ndhA_p5	ndhA	5	AAGGGGTAGATCTATCGGGC	GGGACCAATGTTACGAGCTT	20	20	55.0	50.0	58.1	57.9	5.6	0.0	0.169445	114303	116410	2972-2983	1.000000	80.1