Hotspot

ndhI

SSC · 117055-117561 · 507 bp

Back to Rotheca

Pi 0.49

MarkerSeek score 81.60

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 507
variable_sites: 235
pi: 0.49
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	CTCTAGCCGAGTGTGAGAGA	AGCCACGATTGATTCTCACC	55.0	50.0	58.0	58.0	1131 bp	0.5	59.5
ndhI_p2	TCTCTAGCCGAGTGTGAGAG	AGCCACGATTGATTCTCACC	55.0	50.0	58.0	58.0	1132 bp	0.5	59.5
ndhI_p3	CTCTAGCCGAGTGTGAGAGA	AAGACCCGATCCCAGAAAGA	55.0	50.0	58.0	58.0	1424 bp	0.5	59.4
ndhI_p4	TCTCTAGCCGAGTGTGAGAG	AAGACCCGATCCCAGAAAGA	55.0	50.0	58.0	58.0	1425 bp	0.5	59.4
ndhI_p5	CTCTAGCCGAGTGTGAGAGA	ATGATCCCATACCAGGACGT	55.0	50.0	58.0	57.9	1738 bp	0.5	58.9